PC BASED UV-VIS SPECTROPHOTOMETER

PC BASED UV-VIS SPECTROPHOTOMETER
MAKE DINESH SCIENTIFIC
MODEL DS-SPM-900
DESCRIPTION
A double beam spectrophotometer is an advanced analytical instrument used to measure the absorbance or transmittance of light by a sample at various wavelengths. It works by splitting a light source into two beams: one passes through the sample, and the other passes through a reference. The instrument compares the intensities of these beams to determine the amount of light absorbed by the sample, providing highly accurate and stable measurements. Double beam spectrophotometers are commonly used in laboratories for chemical analysis, molecular biology, and quality control.
FEATURES OF A DOUBLE BEAM SPECTROPHOTOMETER:

 Dual Beam System: Splits the light into two beams one directed at the sample and the other at the reference—allowing for continuous correction of any fluctuations in light intensity, improving accuracy.
 Wavelength Range: Typically covers a broad wavelength range (e.g., UV-visible range from 190 nm to 900 nm), allowing for diverse applications in analyzing different types of samples.
 High Sensitivity: Offers precise absorbance and transmittance measurements, with a high level of sensitivity to detect even small changes in sample concentration.
 Automatic Wavelength Scanning: Many models come with automated scanning features that can sweep across a range of wavelengths, simplifying the analysis process and speeding up data collection.
UPGRADABILITY OPTIONS
Provision for future upgradation with company-fitted accessories:
 Integrating Sphere Diffused Reflecting Accessories
 Temperature Controlled Cell Holder (for cooling/melting)
 Low Volume/Micro Sample Cell Accessories
SOFTWARE: –
 Quantitative analysis measurement modes using time-based and wavelength-based programmable scanning
 Time and wavelength scans for absorbance, sample, reference, %T, and %R
 Windows 10-based software that is easy to use
 Tools for processing data: Data Truncation, Data Smoothing, Derivatives, Arithmetic, Subtraction, Deconvolution, Baseline Correction, Vertical/Horizontal Axis, Peak Picking, Peak Height, Peak Area Measurement, Peak Width Conversion
 Typical operations: Calculating Enzyme Activity, Measuring Film Thickness, Colorimetric Analysis, Macro Command, and Measuring Two Wavelength Time Course
TECHNICAL DETAILS:
MODEL DS-SPM-900
Optical System Double Beam Type
Wavelength Range 190 to 900 nm
Wavelength Accuracy ±0.2 nm at D2 Peak (656.1 nm)
Wavelength Repeatability ±0.05 nm
Light Source D2 Deuterium Lamp & Halogen Lamp
Monochromator Czerny-Turner Type
Grating 1200 Lines/mm
Baseline Stability 0.0004 Abs/hour
Baseline Flatness ±0.0003 Abs (at 200 – 850 nm)
Spectral Bandwidth 0.1 to 10 nm
• L2 to L10 nm
• M1 to M2 nm (Micro cell mode)
Stray Light • 1% (198 nm)
• 0.006% (220 nm)
• 0.006% (340 nm)
• 0.006% (370 nm)
Photometric Repeatability ±0.001 Abs (0 to 0.5 Abs) and ±0.001 Abs (0.5 to 1 Abs)
Photometric Range ±4 Abs
Photometric Accuracy ±0.002 Abs (0 to 0.5 Abs)
• ±0.003 Abs (0.5 to 1 Abs)
• ±0.3 %T
Slew Speed 10,000 nm/min
RMS Noise 0.00003 Abs at 500 nm
Scanning Speed 10 to 4000 nm/min
Preview Scanning Speed 10 to 7000 nm/min
PC Computer PC specifications:
• Intel Core i5 11th Generation
• Keyboard & Mouse
• 17″ LED Monitor
• 8GB RAM DDR4
• 500GB SSD
• Windows 10 Professional 32/64 bit (Licensed)
• All connectivity options essential for UV-Visible Spectrophotometer operation
Detector PMT (Photomultiplier Tube)
Connectivity USB 2.0

ACCESSORIES:
 Connecting wires
 quartz cell (10 mm, 3ml) – 2 pairs, and toolkits