INVERTED RESEARCH MICROSCOPE

INVERTED RESEARCH MICROSCOPE
MAKE DINESH SCIENTIFIC Model DS-IRM-100

DESCRIPTION
An inverted microscope is a specialized optical microscope where the light source and condenser are positioned above the stage, and the objectives are located below the stage. This design is the opposite of a standard microscope, hence the name “inverted.” It is primarily used to observe specimens in containers like petri dishes or culture flasks, which makes it ideal for studying live cells and organisms in their natural environment. Here are the key features:

Key Features:
Optics:
High-quality objectives located below the stage, allowing the examination of thick samples or samples in dishes.

Stage:
Fixed stage designed to hold large or irregularly shaped containers, such as culture dishes or flasks.

Illumination:
Typically uses transmitted light from above, combined with fluorescence or phase contrast methods for enhanced imaging.

Applications:
Ideal for live cell imaging, cell culture observation, and in vitro studies.

Magnification Range:
Usually between 4x and 100x objectives.
2. Objectives (All objectives should be Semi Apochromat / Neo Fluor / Plan Fluor type and fluorescence-compatible):

a. 4X / 5X Objective
Numerical Aperture (NA): 0.13
Working Distance (WD): 17 mm
Suitable for: Brightfield (BF), Darkfield (DF), Fluorescence (FL)

b. 10X Objective
NA: 0.30
WD: 10 mm
Suitable for: BF, DF, Phase Contrast, Fluorescence

c. 20X Objective
NA: 0.45
WD: 6.6 – 7.8 mm
Suitable for: BF, DF, Phase Contrast, Fluorescence

d. 40X Objective
NA: 0.60
WD: 2.5 – 3.0 mm
Suitable for: BF, DF, Phase Contrast, Fluorescence
Note: Listed twice – clarify if 2 units are required.

e. 100X Oil Immersion Objective
NA: 1.30
WD: 0.16 mm
Suitable for: BF, DF, Fluorescence
Additional Requirement: 50 ml immersion oil (in small aliquots)

TECHNICAL DETAILS:

Type Tabletop that is portable and small Fluorescent Inverted Microscope.
Observation Modes:Bright field (BF), Dark field (DF), Phase Contrast (PC), Fluorescence (FL)
Footprint:Small footprint, not exceeding 14 inches
Condenser:All microscope observations can be made using a universal phase condenser.
Image Analysis Advanced image analysis facility (preferably with dark field option)
Stage Manual X-Y-Z axis stage with universal sample holder
Light Source:Advanced transmitted light system with White light LED illumination or 12V intensity control
Nose-piece:5-position nose-piece
Light Sharing Configuration Two-way light sharing between camera port and eye port (100:0, 0:100)
OBJECTIVES
Objectives:4X, 10X, 20X, 40X (BF, Phase Contrast, Fluorescence grade respectively)
Eyepiece:10X Eyepiece with Field of View (FOV) of 22 mm (2 nos.)
Objective a:Semi Apochromat / Neo Fluor / Plan Fluor 4X/5X, NA 0.13, WD 17 mm (for BF, DF, Fluorescence)
Objective b:Semi Apochromat / Neo Fluor / Plan Fluor 10X, NA 0.3, WD 10 mm (for BF, DF, Phase, Fluorescence)
Objective c:Semi Apochromat / Neo Fluor / Plan Fluor 20X, NA 0.45, WD 6.6–7.8 mm (for BF, DF, Phase, Fluorescence)
 
Accessories:All necessary accessories to enable picture transfer from DF to laptop
Fluorescence Light Source LED fluorescence illumination with lifespan of 10,000 hours
Fluorescence Module Manual / Coded / Intelligent fluorescence turret with 4-position turret (3 + 1 filter positions); LED power illumination is user-definable and can be replicated for repeated use of same wavelength.
Fluorescence Filter Sets Includes DAPI, FITC, TRITC; upgradeable to advanced filters
Software Sophisticated imaging software to manage the camera, illumination systems, and every component of the microscope.
Software Features:Time-lapse imaging, multi-channel fluorescent picture capture, volume view, 2D/3D view, morphology, auto counting, auto measurement, Z-stacking, multi-channel imaging, huge image stitching with reference mirror image,
Additional Accessories 1 USB pen drive (32 GB), 1 external hard disk (1 TB)
CAMERA
Camera Type High-resolution advanced microscopic digital color camera
Grade Scientific grade
Connectivity USB 3.0 PC interface
Sensor Type CMOS
Sensor Size 1/1.8 inch
Resolution 15–20 Megapixels (higher preferred)
Pixel Size At least 3.45 µm × 3.45 µm
Bit Depth 12-bit
Live Display Speed Greater than 25–30 FPS at 1K resolution
Imaging Modes Supported Fluorescence, Phase Contrast, Dark Field (DF), DIC, Bright-field
Included Accessories USB 3.0 cable, software, C-mount adapter, microscope adapters (if required)

Image Capturing & Storing Device
•Compact portable laptop with:
•Intel Pentium i7 processor
•1TB HDD
•12 GB RAM
•15” or 21” high-resolution TFT color monitor
•Multi-user MS Office
•Multi-user antivirus software
•Suitable original operating software
•Multimedia keyboard
•1 GB graphics card