GEL DOCUMENTATION SYSTEM
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DESCRIPTION
A specialized lab tool called a Gel Documentation System is used to see and record protein and nucleic acid samples that have been separated using gel electrophoresis. Labs that study biochemistry and molecular biology frequently use this approach. A high-resolution camera, image software, and a UV or white light transilluminator are usually the main parts of a gel documentation system.
TRANSILLUMINATOR:
- To illuminate the gels, the system is fitted with a transilluminator that emits either white light or ultraviolet (UV) radiation. White light works well for protein gels, however UV light is typically utilized for DNA and RNA gels.
CAMERA:
- To take crisp, in-depth pictures of the segregated biomolecules inside the gel, a high-resolution camera is built into the device. The particular wavelengths that fluorescent dyes, which are frequently employed to stain DNA, RNA, or proteins, emit are detectable by the camera.
FILTERS:
- Gel Documentation Systems frequently come with replaceable filters that can be used to catch particular light wavelengths, making it possible to see various fluorophores.
IMAGE SOFTWARE:
- Specialist imaging software is used to process and analyze the acquired images. With the aid of this software, researchers may measure and record outcomes, such as the length and width of protein lanes or DNA bands on the gel.
DOCUMENTATION:
- Film-based photography and other conventional techniques are no longer necessary thanks to Gel Documentation Systems, which make it easier to digitally record gel electrophoresis data. Gel pictures can be electronically saved, arranged, and shared by researchers.
VERSATILITY:
- Certain Gel Documentation Systems are made flexible enough to meet varied experimental needs by accommodating a range of gel types and sizes.
HIGH-SPECTRUM CAMERA:
- Clear and detailed photos of the separated biomolecules in the gel are captured using an integrated high-resolution camera.
SOURCES OF EXCITATION AND FILTERS:
- Different fluorescent dyes used to stain DNA, RNA, and proteins can be accommodated by interchangeable filters and numerous excitation sources. Because of this adaptability, researchers can see different kinds of samples.
FEATURES :
- No light seepage Darkroom
- Dimensions outside: 44x40x72cm
- CCD Lens
- CCD Science
- 5 Megapixel, 2592*1944 resolution
- 16-bit A/D (65,536 greyscales)
- High QE: 75%
- Noise in reads: 5.1e-RMS
- SNR: 70.1 dB
- Sensitivity (2.7): EB < 10 pg DNA staining
- Imported 2/3-Inch Computer, F1.2 Automated Lens
- Aperture and Focus Numbers
- The usual filter for gel documents is 590 nm.
- White LED * 2 Epi-white light Lamp
- Standard UV transmission: Wavelength: 302 nm; Area: 21 x 26 cm
- Optional white LED light, 21 x 19 cm
- Optional Blue LED: 21 x 19 cm
- UV shield as a standard feature
- Gel Doc program
- Software for analysis
- Application (often included)
TECHNICAL DETAILS:
MODEL | DS-GDS-938 |
Compact benchtop instrument | System with the facility to view and quantify images of fluorescent DNA, RNA, and protein and colorimetric gels. UV and visible light transillumination. Motorized zoom lens. |
UPS | Online UPS compatible with machine and workstation (1 KVA) . |
Power Supply | 210-240 V/50 Hz. |
CAMERA | High-speed USB technology for faster image capture and download. Autofocus configuration. |
Data Acquisition | 12-bit and 6000 gray levels. |
Field of View | At least 10 cm x 14 cm. |
Control System | Automatic and inbuilt control of all system operations, including image capture, camera exposure, lens setting, darkroom, light, and filter controls. |
CCD Camera | 8-megapixel, 16-bit Scientific-Grade CCD Camera cooled to -25°C absolute and regulated. Fully motorized camera, lens, and darkroom controls. |
Lens | High-quality lens (0.9USM) with motorized optics. |
Filter Wheel | At least four positions motorized filter wheel for best optics. |
Excitation Source | Trans-UV, 254nm, 365nm. Wide transillumination area. |
Work Station | · Branded laptop with i7 dual-core Processor, 4GB RAM, 1TB HDD, DVD R/W Drive, separate graphics card with 17″ TFT monitor.
· Compatible with Windows and Microsoft Office. |
Light Sources | 302nm or 365nm UV transilluminator, LED-white lights, Trans-white light via white light conversion screen. |
Darkroom | Light-tight darkroom with UV safety switch. |
Software | · Imaging and analyzing 1-D electrophoretic gels, dot blots, slot blots, and colony counts. Rapid molecular weight determinations.
· Band/lane matching with comparative dendrogram creation. · Background subtraction correction of gradient gels. 3D viewer facility for analysis of closely spaced bands. |
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