HORIZONTAL  ELECTROPHORESIS SYSTEM

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 DESCRIPTION

A horizontal electrophoresis system is designed to separate DNA, RNA, or proteins in a horizontal gel medium, typically using agarose or polyacrylamide gels. The system is widely used in molecular biology laboratories for gel electrophoresis, a technique that allows for the separation and analysis of macromolecules based on their size and charge.

Key Components of a Horizontal Electrophoresis System:

GEL TRAY:

• Holds the agarose or polyacrylamide gel in which samples are loaded. It is positioned horizontally within the unit.

BUFFER CHAMBER:

• Contains the buffer solution (e.g., TAE, TBE), which maintains pH and provides ions to conduct the electrical current through the gel.

ELECTRODES:

• Positioned at both ends of the buffer chamber, these generate an electric field that drives the movement of charged molecules (e.g., DNA or proteins) through the gel.

POWER SUPPLY:

• Delivers a constant voltage or current to the electrophoresis system, allowing the macromolecules to migrate through the gel matrix.

SAMPLE WELLS:

• Created using a comb, these wells are where the samples are loaded before the electric field is applied. Each well holds a small amount of sample for separation.

 LID AND SAFETY FEATURES:

• The system typically includes a transparent lid that prevents users from coming into contact with the electrodes or buffer during operation. Many systems also have safety features like automatic shutoff when the lid is removed.

COMMON FEATURES AND SPECIFICATIONS:

• Gel Format: Typically mini or large gels, depending on the system size.
• Comb Sizes: Available in various well sizes (e.g., 8-well, 12-well combs) for different sample loading needs.
• Buffer Volume: The amount of buffer required to submerge the gel for proper electrophoresis.

TECHNICAL DETAILS: