HORIZONTAL ELECTROPHORESIS SYSTEM
MAKE DINESH SCIENTIFIC
DESCRIPTION
A horizontal electrophoresis system is designed to separate DNA, RNA, or proteins in a horizontal gel medium, typically using agarose or polyacrylamide gels. The system is widely used in molecular biology laboratories for gel electrophoresis, a technique that allows for the separation and analysis of macromolecules based on their size and charge.
Key Components of a Horizontal Electrophoresis System:
GEL TRAY:
- Holds the agarose or polyacrylamide gel in which samples are loaded. It is positioned horizontally within the unit.
BUFFER CHAMBER:
- Contains the buffer solution (e.g., TAE, TBE), which maintains pH and provides ions to conduct the electrical current through the gel.
ELECTRODES:
- Positioned at both ends of the buffer chamber, these generate an electric field that drives the movement of charged molecules (e.g., DNA or proteins) through the gel.
POWER SUPPLY:
- Delivers a constant voltage or current to the electrophoresis system, allowing the macromolecules to migrate through the gel matrix.
SAMPLE WELLS:
- Created using a comb, these wells are where the samples are loaded before the electric field is applied. Each well holds a small amount of sample for separation.
TECHNICAL DETAILS:
| MODEL | DS-HES-10 |
| Length of the Gel tray (cm) | 10 cm |
| Width of the Gel tray (cm) | 7 cm |
| Number of well combs | 8 |
| Purpose | The term “horizontal electrophoresis system” describes equipment that is frequently used in molecular biology and cell biology labs to separate DNA and RNA based on their size, mass, and/or electrical charge in agarose gels. |
| Provision of uv transparent gel trays with florescent ruler | Yes |
| System provided with three sets of output terminals to deliver constant voltage or current | Yes |
| Led display for voltage, current, power and time | Yes |
| Safety feature | Includes no load detection, sudden load change detection, overload/short circuit detection, and overvoltage detection |
| Thickness of comb (mm) | 1 |
| Sample Capacity | 1 |
| Construction of Unit (Moulded/Fabricated) | Single Moulded |
| Type of Electrodes (Fixed/Removable) | Removable |
| buffer volume (ml) | 1 |
| Provision of gel casting gates for sealing the tray | Yes |
| Easy replacement of electrode assemblies and lid removal to avoid buffer spillage | Yes |
| Additional | |
| Gel Tray Size | 7-8 cm (Width) x 7-10 cm (Length) |
| Sample Throughput | 1 to 30 samples |
| Well Comb Options | 8-well or 15-well comb (other sizes permissible) |
| Base Buffer Volume | 200 to 500 ml |
| Gel Tray Size | 23-25 cm (L) x 13-15 cm (W) |
| Sample Throughput | 1200 samples |
| Base Buffer Volume | Range of 600 to 1800 ml |
| Well Comb Options | 15-well or 20-well comb (other sizes allowed) |
| Medium Tank | |
| Medium Tank | · 12-15 cm (L) x 10-14 cm (W)
· Between 1 and 100 samples · Range of 400 to 1200 ml · 15-well or 20-well comb (other sizes allowed) |
| UV Transparent Gel Tray | Includes an integrated fluorescent ruler and safety lid |
| Compatibility | The same buffer tank accommodates different gel tray sizes |
| UV Transparent Gel Tray | Includes an integrated fluorescent ruler and safety lid |
| Adjustable Gel Caster | External gel caster with adjustable settings |
| Bubble Leveller | Included for even gel casting and running |
| Lid with Integrated Cables | Allows direct connection to the power pack |
| POWER SUPPLY | |
| Voltage Range | 10 – 300 V |
| Current Range | 0 – 700 mA |
| Power Output | 75 – 150 W |
| Additional Features | Pause and resume function |
| Display | LED or TFT-LCD |
| Output Type | Constant current, constant voltage |
| Timer | 0 – 999 minutes |
| Number of Outputs | 3 |
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