SINGLE BEAM SPECTROPHOTOMETER

MAKE DINESH SCIENTIFIC

DESCRIPTION

A single-beam spectrophotometer is an instrument used to measure the intensity of light absorbed by a sample at specific wavelengths. Here is a detailed description of its components and working principles:

Components

• Light Source:

Provides a consistent and stable light beam, typically a tungsten or deuterium lamp for visible and UV range measurements, respectively.

• Monochromator:

Separates the light into its component wavelengths. This can be achieved using a diffraction grating or a prism to select the desired wavelength for measurement.

• Sample Holder/Cuvette:

Holds the sample in a transparent container, typically made of glass or quartz, allowing the light to pass through the sample.

• Detector:

Measures the intensity of light passing through the sample. Common detectors include photodiodes, photomultiplier tubes, or CCD arrays.

• Display/Readout:

Shows the absorbance or transmittance values, often as a digital readout on an LCD or LED screen.

• Wavelength Selector:

Allows the user to choose the specific wavelength at which the measurement will be taken.

• Sample Compartment:

A chamber where the cuvette containing the sample is placed. It often includes a lid to block external light and ensure accurate readings.

• Calibration and Baseline Adjustment:

Functions for setting a baseline with a reference (usually a blank or solvent) and calibrating the instrument to ensure accurate measurements.

• Data Output:

Options to connect to a computer or printer for data recording and analysis. Modern spectrophotometers may include USB or other digital interfaces.

• User Interface:

Buttons, knobs, or touch screens for controlling the instrument and setting parameters such as wavelength and measurement mode.

• Software Integration:

Some models come with software for data analysis, graphing, and reporting, enhancing the functionality and ease of use.

• Portability:

Some single beam spectrophotometers are designed to be portable for fieldwork, featuring compact sizes and battery operation.

• Temperature Control:

Optional feature to maintain consistent temperature during measurements, which is crucial for certain types of samples.

• Maintenance Alerts:

Indicators for lamp replacement, calibration reminders, and other maintenance needs to ensure the instrument

remains in optimal working condition.

• Safety Features:

May include automatic shut-off, protective covers, and interlocks to prevent exposure to harmful UV light.

TECHNICAL DETAILS: